Abstract
During the past few years a number of laboratories have developed very efficient mass spectrometry methods for analyzing proteins separated by 2-D PAGE; however, there are considerable difficulties associated with gel based methods. It can be very difficult to recover some proteins from the gel and "in-gel" digestion efficiency of some proteins is very low. In an effort to circumvent the difficulties associated with gel based methods we have developed two non-gel mass spectrometry methods for analyzing protein mixtures: (1) direct analysis of complex protein mixtures (whole cell lysates) with high resolution time-of-flight (TOF) MS and matrix-assisted laser desorption ionization (MALDI) and (2) high pressure MALDI (ionization of mixtures)-ion mobility (separation)-TOFMS. The two experimental approaches and results from each will be presented. The primary focus of the presentation will be on peptide mass mapping protein data base (PDB) searching for protein identification, but results from studies using the same experimental methods but aimed at understanding protein folding (beta-lucerifase) will also be presented.
 

Background
David H. Russell, B. Sc. (1974) Chemistry, University of Arkansas-Little Rock; Ph. D. (1978) University of Nebraska (Dr. Michael L. Gross). Dr. Russell spent two years at Oak Ridge National Laboratory (1978-1980). He is currently Professor of Chemistry and Director of the Laboratory for Biological Mass Spectrometry at the Texas A&M University. He is also Director of the Biological Mass Spectrometry Core Research Laboratory, NIEHS Center for Environmental and Rural Health, College of Veterinary Medicine, Texas A&M University.

Dr. Russell's research focuses on three main areas: gas-phase ion chemistry, biological mass spectrometry, and instrumentation development. The ion chemistry studies involve both unimolecular and bimolecular reaction chemistry. Active research projects include studies of unimolecular, thermally-induced, and photon-induced dissociation reactions of small organic ions, studies of ion-molecule reactivity of metal ions with polar organic molecules, and experimental and computational approaches to determination of structures of organo-metal ion complexes. Biological mass spectrometry research includes studies of protein folding using MALDI in combination with H/D exchange and pulsed alkylation, the use of laser photodissociation and tandem mass spectrometry to sequence peptides, and the development of high resolution time of-flight (HR-TOF-MS) methods for identification and characterization of proteins present in complex mixtures, e.g., whole cell lysates. The major focus of instrumentation development research is in the area of ion mobility, Fourier-transform ion cyclotron resonance (FTICR) and TOF-MS. We are currently developing two novel ion mobility instruments: (1) a 7-tesla FTICR ion mobility apparatus for structural studies (volume-to-charge ratios) of small ions (m/z < 1500 da.) and structures of solvated ions, and (2) an ion mobility-TOF instrument for rapid (0.1-1 millisecond) separation and sequencing of peptides generated by proteolytic digestion of proteins.
 
 

Meeting details

Date:	Wednesday	January 26, 1999
Time:	6:00 pm	Social hour, registration
	7:00 pm	Dinner ($25 for BAMS members; $35 non-members)
	8:00 pm	Presentation (free)
Dinner:	Choice of:	Chicken Chausseur (mushroom and wine sauce with rice and vegetables on the side)
		Veggie Penne Pasta with Basquaise sauce
	includes	Wine, Ice cream, Coffee and tea
Cost:	$25.00	BAMS members.  Reservations required by noon Friday 1/21/00
	$35.00	non-BAMS members.  Reservations required by noon Friday 1/21/00
	$15.00	Students only.  Reservations required by noon Friday 1/21/00

Maps & directions
Basque Cultural Center
599 Railroad Ave.
South San Francisco
650-583-8091
 

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00pm for cocktails, dinner at 7:00pm, and lecture at 8:15pm.
 

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Last update: 1/12/00.