Abstract
Since the advent of the Human Genome Project, we have witnessed unparalleled growth in the demand for characterizing biological expression within living systems.  Differential biology and cellular diversity is being explored on both genotypic and phenotypic levels.  While the recent past has witnessed significant advances in DNA and RNA analysis, advances in the analysis of proteins have primarily focused upon hyphenating electrospray or maldi ionization mass spectrometry with established separation / isolation approaches such as 2-dimensional gel electrophoresis.

In 1998, we described a new methodology for rapid, facile analysis of protein expression, biomarker discovery, and proteomic investigation based upon ProteinChip™ array technology combined with Laser Desorption Ionization Time-of-Flight Mass Spectrometry (LDI-TOF).  In this manner, complex biological mixtures are exposed to specifically modified ProteinChip surfaces in a fashion that allows differential protein extraction and retention based upon well-established molecular recognition and intermolecular binding mechanisms.  Retained proteins are detected using the LDI-TOF analytical technique.  The relative ease, speed, and power of this approach has been shown to rapidly elucidate biomarker candidates and other proteins of interest, often requiring only a few days of effort.

In this talk, we depict the ProteinChip platform as a combined protein separation, characterization, identification, and assay tool.  We have developed ProteinChip based assay techniques that perform enrichment and purification of target proteins from complex biological media.  Target proteins are subsequently characterized and identified by exposing them to an array of chemical and/or enzymatic modifications and then monitoring the resultant structural changes using differential ProteinChip LDI-TOF mass spectrometry.  Identified shifts in molecular weight or ion population profiles are reconciled with known mechanisms of chemical or enzymatic activity to provide information regarding post-translational modifications, peptide composition, partial or complete primary sequence, and data-base mediated protein identification.  Furthermore, we introduce the technique of ProteinChip Laser Desorption Ionization Quadrupole Time-of-Flight Mass Spectrometry (ProteinChip LDI-QTOF).  The enabling power of ProteinChip LDI-QTOF to identify protein candidates even in the presence of contaminant species will be demonstrated.
 

Background
Currently Mass Spectrometry Research Manager for Ciphergen Biosystems Inc. Previously worked as MALDI-TOF Product Manager and Principal MALDI Scientist for the Hewlett Packard Company; Vice President of Marketing and Research at Linear Scientific / Biomolecular Separations Inc.; Capillary Electrophoresis R/D Group Leader for Spectra Physics Incorporated; Applications Manager for Linear Instruments.

Education: BS Chemistry at Ohio State University and BS Cellular Biology at the University of Nevada Reno, Three years medical training at the University of Nevada School of Medicine, and PhD studies in Physical Biochemistry at the University of Nevada, Reno, Department of Biochemistry.

Current Interests: Biomass spectrometry, SELDI, MALDI, Time-of-Flight Mass Spectrometry, micro fluidics and microseparation techniques.Numerous patents and publications in MALDI, Time-of-Flight Mass Spectrometry, Capillary Electrophoresis, UV/Visible Spectroscopy.
 
 

Note: Do we have your correct e-mail address?  Quite a number of bounced e-mails implies that our mailing list isn't up to date.  If you haven't been receiving our e-mail announcements (or they are coming via an undesirable e-mail), please contact Lori (zeller2@llnl.gov) and she will correct our e-mail list.
 

Meeting details

Date:	Thursday	June 29, 2000
Time:	6:00 pm	Social hour, registration
	7:00 pm	Dinner ($25 for BAMS members; $35 non-members)
	8:00 pm	Presentation (free)
Dinner:	Choice of:	Chicken Chausseur (mushroom and wine sauce with rice and vegetables on the side)
		New York Sirloin (with appropriate side dish ...)
		Veggie Penne Pasta with Basquaise sauce
	includes	Wine, Ice cream, Coffee and tea
Cost:	$25.00	BAMS members.  Reservations required by noon Friday 6/23/00
	$35.00	non-BAMS members.  Reservations required by noon Friday 6/23/00
	$15.00	Students only.  Reservations required by noon Friday 6/23/00

Maps & directions
Basque Cultural Center
599 Railroad Ave.
South San Francisco
650-583-8091
 

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00pm for cocktails, dinner at 7:00pm, and lecture at 8:15pm.
 

If you have suggestions for a meeting topic, or know of a prominent mass spectroscopist who will be visiting the bay area, please contact our program chair.
 

BAMS is pleased to post Employment postings for our members.

To become a member, please fill out and mail in a membership form.
BAMS has been fortunate to have had many excellent speakers in our short history.
If you have suggestions for a meeting topic please contact our program chair.
If you have questions or comments about BAMS, please contact one of the BAMS officers.
Please thank our corporate sponsors who help keep BAMS membership and dinner costs low.
Here are some internet links that may be of interest to BAMS members.
BAMS treasurer's report for 1999 is available here.
 

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Last update: 6/20/00.