Abstract
A new multiplexing technique for screening combinatorial libraries of enzyme inhibitors using FTICR and immobilized enzymes will be presented along with a new method for the calculation of kinetic constants.  Over 1,000 compounds have been screened for several important sulfotransferase enzymes, including estrogen sulfotransferase.  As inhibitors are identified, they are subjected to a novel strategy for determining Ki kinetic parameters using an ion trap mass spectrometer.  This method allows for the calculation of Km and Vmax as well as Ki and thus enables the investigator to track the kinetic mechanisms regarding competitive or non-competitive binding.  Additionally, the non-covalent complexes between substrate/inhibitor and enzyme are generated using FTICR and structure specificity and folding characteristics are probed.

Background
Dr. Julie Leary is Professor of Chemistry at UC Berkeley having received her Ph.D. with Klaus Biemann from MIT. She then had a one year postdoctoral appointment at Woods Hole Oceanographic and the MIT Mass Spectrometry Resource Facility before moving to Berkeley.  She is recipient of the 2000 Biemann Medal and Sigma Xi awardee.  Her current area of research interest includes carbohdrate analysis from bacteria and pursuit of enzymatic processes using a multiplexing approach.  Julie keeps busy outside of her career with two teenaged children, a husband and classes in Taiko to keep her sane.

Meeting details

Date:	Tuesday	January 15, 2002
Time:	6:00 pm	Social hour, registration (no-host cocktails)
	7:00 pm	Dinner
	8:00 pm	Lecture
Dinner:	Choice of:	Grilled Breast of Chicken with Button Mushrooms and Lemon Butter Sauce
		Grilled Red Snapper Brudetto (with Seasoned Tomato Sauce)
		Pasta Primavera
	includes	(various side dishes & dessert)
Cost:	$25.00	BAMS members.  Reservations required by noon on Friday January 11, 2002
	$35.00	Non-members.  Reservations required by noon on Friday January 11, 2002
	$15.00	Students only.  Reservations required by noon on Friday January 11, 2002

Note: 2002 dues need to be paid to obtain member price.  Dues ($20) may be paid while registering for dinner.

Maps & directions
Francesco's
8520 Pardee Drive at Hegenberger Road, Oakland, CA 94621
(510) 569-0653
Yahoo map
 
 

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00pm for cocktails, dinner at 7:00pm, and lecture at 8:15pm.

If you have suggestions for a meeting topic, or know of a prominent mass spectroscopist who will be visiting the bay area, please contact our program chair.

As a service to our members, we post Employment postings.

To become a member, please fill out and mail in a membership form.
BAMS has been fortunate to have had many excellent speakers in our short history.
If you have suggestions for a meeting topic please contact our program chair.
If you have questions or comments about BAMS, please contact one of the BAMS officers.
Please thank our corporate sponsors who help keep BAMS membership and dinner costs low.
Here are some internet links that may be of interest to BAMS members.
 

If you have comments or suggestions for this web site, please email me at hugh-gregg@llnl.gov

Last update: 1/3/02.