Abstract
Introduction
In the shotgun sequencing approach, proteins extracted from cells or tissues are digested directly with trypsin.  The digested mixture can then be separated by capillary reversed-phase HPLC on-line with mass spectrometry (LC-MS).  Thus, matching the database with the experimental MS/MS spectra from a LC-MS run can identify many different proteins from a single sample.

Methods
Laser Capture Microdissection is a powerful tool for isolating pure cell populations from complex tissue.  This cell capture is accomplished on the PixCell II LCM system through the attachment of desired cells to laser activated thermoplastic film.  We demonstrated the mating of these two technologies by testing a model system. Approximately, 10,000 SKBR3 cells were captured and proteins extracted from the capture device by incubation with a guanidine isothiocyanide based buffer containing beta-mercaptoethanol.  The extracted proteins were reduced, alkylated, and then digested with the following enzymes: Lys-C, then trypsin, with a series of dilution protocols.  The entire digest mixture was loaded directly on a capillary reversed-phase column following by a series of washing procedures and analyzed by on-line LC/MS (ion trap mass spectrometer).  Protein identifications were made with Bioworks (Sequest) and a probability based software.

Preliminary Data
Cancer biomarkers, including the Her2 receptor, and a substantial number of other proteins were preliminary identified by this approach.  Initially, more than 200 proteins were identified with 1 or more peptides and approximately 130 proteins were identified with an additional filter of Xcorr >/ = 1.5 and an uncertainty value of 1/1,000,000 or P value < 0.0001%.  This approach may pave the way in the future to the study of proteomics of small tissue samples.

Background
EDUCATION & PROFESSIONAL POSITIONS
2001  Editor–in-Chief, Journal of Proteomic Research, Amer. Chem. Soc.
2000  Vice President Proteomics Development, ThermoQuest Inc.
1996   Professor of Chemical Engineering, Adjunct, Yale University
1994-present  Principal Laboratory Scientist, HP Laboratories.
1985-1994  Director, Analytical Chemistry & Staff Scientist, Genentech Inc.
1984   Visiting Scientist at Bureau of Drugs, FDA, Washington DC
1972-1985  Reader in Chemistry, Biochemistry, Massey University, New Zealand.
1963-1970 B.Sc.1966, Ph.D.1970, in Organic Chemistry and Biochemistry, D.Sc.1993,  University of Adelaide, South Australia

CONTRIBUTIONS TO INDUSTRY-STANDARDS  & ORGANIZATIONS
• Advisory board of San Jose State Univ.,Univ. of Kansas, Barnette Inst., Boston,  Institute of Analytical Chemistry, Innsbruck, Austria
• Permanent Scientific Committee for HPCE, HPLC, WCBP Symposium Series
• Scientific Committee for APCE'96, HPCE 96, PBA 96, HPLC ‘95, ‘97, ‘98, ‘99, 2000, HPCE Kyoto’97, Drug Analysis ‘98
• Californian Separation Science Society, President, 1990 - present
• Past and present member of 12 Journal Boards including Analytical Chemistry (1992-2001) and Journal of Chromatography
• Chairman of the HPLC96; HPCE97&2000 and The Analysis of Well Characterized Biotechnology Pharmaceuticals”, 1997 &1998
• Chairman of the United Way Campaign for HP Labs, 1997

HONORS AND AWARDS:
• Martin Gold Medal in Separation Science (British Chromatographic Society, 2000)
• Barnett Lecturer, NE University, Boston (1992)
• Honorary Fellow of Massey University, New Zealand
• Walter Burfitt Prize and Research medal, Royal Society of New South Wales for Pure or Applied Scientific Research (1984).

PUBLICATIONS:
Over 160 scientific publications, including 114 manuscripts accepted in International Journals, 34 review chapters, 7 books and 9 patents, as well as over 150 invited presentations at International Scientific Meetings

Meeting details

Date:	Tuesday	May 28, 2002
Time:	6:00 pm	Social hour, registration (no-host cocktails)
	7:00 pm	Dinner
	8:00 pm	Lecture
Dinner:	Choice of:	Marinated Chicken Dinner
		Vegetarian Dinner
	includes	various side dishes & dessert
Cost:	$25.00	BAMS members.  Dinner reservations must be made by noon Friday May 24, 2002
	$35.00	Non-members.  Dinner reservations must be made by noon Friday May 24, 2002
	$15.00	Students only.  Dinner reservations must be made by noon Friday May 24, 2002

Note: 2002 dues need to be paid to obtain member price.  Dues ($20) may be paid while registering for dinner.

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The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00 pm for cocktails, dinner at 7:00 pm, and lecture at 8:15 pm.

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Last update: 5/14/02.