Abstract
This talk will describe some of our LC/MS/MS work in both high and low throughput applications.  The high-throughput applications involve use of multi-dimensional separations at the protein and peptide level coupled to an ion trap mass spectrometer for detecting differential protein expression in synovial fluid for the purposes of finding biomarkers for rheumatoid arthritis. Protein Identification is accomplished from the MS/MS scans and quantitation is derived from the peptide ion current in the intervening MS survey scans. The low throughput applications involve identifying in vivo phosphorylation sites from immunoprecipitation of epitope-tagged proteins by LC/MS/MS in multiple reaction monitoring mode, following comprehensive mapping of phosphorylation sites from in vitro kinase reactions using purified kinase and substrates. The merits of LCQ and QTrap mass spectrometers for these experiments will be illustrated.

Background
Karl has been a scientist in the Department of Discovery Technology at Millennium Pharmaceuticals Inc. in Cambridge, MA since 1997. He is engaged in the development of high-throughput LC/MS/MS techniques for studying differential protein expression, identifying protein complex constituents, and in vivo and in vitro phosphorylation site identification.  He did his graduate training in mass spectrometry at the University of California San Francisco (UCSF) under the direction of Professor Alma Burlingame, though neglected to complete his PhD dissertation. While at UCSF he co-developed the ProteinProspector software package with Peter Baker for interpreting mass spectrometry data generated from proteomics experiments, and used these tools to study differentially expressed proteins isolated via 2D-PAGE for the pregnancy disorder pre-eclampsia and the response of tumor cell lines to treatment with cytokines. His interest in mass spectrometry was kindled while performing research under the direction of Dr. John Stults in the Protein Chemistry department at Genentech Inc. from 1989-1991. He earned a B.S. degree in Chemistry at the California Institute of Technology in 1986.

Meeting details

Date:	Tuesday	July 30, 2002
Time:	6:00 pm	Social hour, registration (no-host cocktails)
	7:00 pm	Dinner
	8:00 pm	Lecture
Dinner:	Choice of:	Vegetarian Pasta
		Salmon with Champagne Sauce
		Filet Mignon with Shallot Sauce
	includes	(various side dishes & dessert)
Cost:	$25.00	BAMS members.  Reservations required by noon on Friday July 26, 2002
	$35.00	Non-members.  Reservations required by noon on Friday July 26, 2002
	$15.00	Students only.  Reservations required by noon on Friday July 26, 2002

Note: 2002 dues need to be paid to obtain member price.  Dues ($20) may be paid while registering for dinner.

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The Basque Cultural Center
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South San Francisco, CA 94080
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The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00 pm for cocktails, dinner at 7:00 pm, and lecture at 8:15 pm.

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Last update: 7/26/02.