Abstract
Protein phosphorylation is probably the most abundant reversible post translational modification in the human proteome, regulated by 518 protein kinases and 65 protein phosphatases.  Although it is an abundant PTM, it is not the easiest to study by conventional peptide mass spectrometry methods, as the combination of low stochiometry and poor ionization leads to a low representation of phosphopeptides in LC-MS based database searches.

Utilizing the selective scanning features of a 4000 Q-Trap mass spectrometer, such as precursor ion scanning and multiple reaction monitoring (MRM), protein phosphorylation site identification by LC-MS is becoming more routine.  The rapid duty cycle of both precursor ion scanning and MRM permits phosphopeptide detection and sequencing by MS/MS in an LC-MS workflow.  Examples of phosphorylation site identification from endogenous proteins from either cell line or transgenic animal sources and examples of multiply phosphorylated proteins (>18 sites per protein) will be given.

The effectiveness of mass spectrometry to identify protein phosphorylation sites will be compared to solid phase Edman sequencing of 32P labeled phosphopeptides, which we routinely run in our laboratory.  The nature of phosphopeptides that are commonly missed by LC-MS techniques will be discussed and possible solutions to this problem.

Background
(not available)

Meeting details

Date:	Tuesday	January 18, 2005
Time:	6:00 pm	Social hour, registration (no-host cocktails)
	7:00 pm	Dinner
	8:15 pm	Lecture
Dinner:	featuring:	Marinated Chicken Breast (rice pilaf, assorted vegetables and asparagus with a sweet corn ragout)
		Charbroiled Pacific Salmon (rice pilaf, grilled asparagus with seasonal vegetables and tomato-corn relish)
		Vegetarian Lasagna (squash, zucchini, spinach, mushroom with Ricotta and Parmesan cheese)
	includes:	Salad and various desserts
Cost:	$30.00	BAMS members.
	$40.00	Non-members.
	$60.00	BAMS membership plus dinner cost
	$15.00	Students only.
		Dinner reservations required by noon on Friday February 11, 2005

Note: 2005 dues need to be paid to obtain member price.  Dues ($30) may be paid when registering for dinner.
If you are unsure if you are paid up for 2005, please email David Kage.

Maps & directions
Crowne Plaza
1221 Chess Dr.
Foster City, CA 94404
(650) 570-5700
maps.yahoo.com directions

Did you see or hear about the BAMS talk by Professor Murayama entitled "What is Mass?"  What is it we are measuring after all?  Check it out!  Go to ctp.berkeley.edu and click on "Popular Talks & Articles" for a QuickTime file.

As a service to our members, we post Employment postings.

As a trial service to our members, we are now posting Situations Wanted.  (Note: BAMS isn't a career center and will only post brief positions wanted for BAMS members only).

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00 pm for cocktails, dinner at 7:00 pm, and lecture at 8:15 pm.

If you have suggestions for a meeting topic, or know of a prominent mass spectrometrist who will be visiting the bay area, please contact our program chair.

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BAMS has been fortunate to have had many excellent speakers in our short history.
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Here are some internet links that may be of interest to BAMS members.
 

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Last update: 2/7/2005