Abstract
The curved-field reflectron provides a means for achieving very high energy collisions (up to 20 keV in the laboratory frame) on a tandem (TOF/TOF) time-of-flight mass spectrometer. Moreover, because deceleration of the precursor ions and reacceleration (lifting) of the product ions is not required, all of the post-source decay products are recovered in the mass spectra and recorded at the same flight times as their corresponding CID products, offering the opportunity for comparison of these modes of ion activation. Both PSD (laser induced) and high energy CID are thought to provide remote site fragmentation that may reduce the losses of modifying groups on peptides and enable the identification of post-translational modification sites.

A modified Kratos AXIMA CFR was used to determine acetylation sites in histone acetyltransferase (HAT) as part of our participation in the Networks and Pathways of Lysine Modifications project.  N-terminal sulfonation was used to produce complete and exclusive series of y-ions that also enabled us to determine ubiquitylation sites on CHIP (C-terminus of Hsp-70 interacting protein) and on the polyubiquitin chain.

Background
Robert J. Cotter is Professor of Pharmacology and Molecular Sciences at the Johns Hopkins School of Medicine.  He also holds joint appointments in the Department of Biophysics and Biophysical Chemistry and at the Johns Hopkins Applied Physics Laboratory. He received his BS in Chemistry at the College of the Holy Cross in Worcester, Massachusetts in 1965 and his PhD in Physical Chemistry at the Johns Hopkins University in 1972.  He is a former President of the American Society for Mass Spectrometry, and author of the text: Time-of-Flight Mass Spectrometry: Instrumentation and Applications in Biological Research, published by ACS Books/Oxford.  He has had a long time interest in the development of time-of-flight mass spectrometers, including miniaturization as both bioagent detectors and point-of-care diagnostics.  His laboratory has focused on several biological structural areas, including lipid A and lipopolysaccharides from gram negative bacteria, class I antigens and molecular mimicry in autoimmune response, phosphorylation sites in viruses, and more recently on protein biomarkers associated with heart failure and found in the albuminome.

Meeting details

Date:	Tuesday	May 10, 2005
Time:	6:00 pm	Social hour, registration (no-host cocktails)
	7:00 pm	Dinner
	8:00 pm	Lecture
Dinner:	featuring:	Veal Roast
		Salmon with Champagne Sauce
		Vegetarian Pasta
	includes:	Salad, side dishes and desserts
Cost:	$30	BAMS members.
	$40	Non-members.
	$60	BAMS membership plus dinner cost
	$15	Students only.
	$300	Corporate Sponsors
		Dinner reservations required by noon on Friday May 6, 2005

*Note:  2005 dues need to be paid to obtain member price.  Dues ($30.00) may be paid while registering for dinner.  If you are unsure of your 2005 Membership status, please email Carol Soppe (carol.soppe@thermo.com) or Shanhua Lin (shanhua.lin@menlo.ppdi.com).

Maps & directions
The Basque Cultural Center
599 Railroad Avenue
South San Francisco, CA 94080
maps.google.com directions

Did you see or hear about the BAMS talk by Professor Murayama entitled "What is Mass?"  What is it we are measuring after all?  Check it out!  Go to ctp.berkeley.edu and click on "Popular Talks & Articles" for a QuickTime file.

As a service to our members, we post Employment postings.

As a trial service to our members, we are now posting Situations Wanted.  (Note: BAMS isn't a career center and will only post brief positions wanted for BAMS members only).

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00 pm for cocktails, dinner at 7:00 pm, and lecture at 8:15 pm.

If you have suggestions for a meeting topic, or know of a prominent mass spectrometrist who will be visiting the bay area, please contact our program chair.

To become a member, please fill out and mail in a membership form.
BAMS has been fortunate to have had many excellent speakers in our short history.
If you have suggestions for a meeting topic please contact our program chair.
If you have questions or comments about BAMS, please contact one of the BAMS officers.
Please thank our corporate sponsors who help keep BAMS membership and dinner costs low.
Here are some internet links that may be of interest to BAMS members.
 

If you have comments or suggestions for this web site, please email me at hugh-gregg@llnl.gov

Last update: 4/27/2005