The San Francisco Bay Area Mass Spectrometry (BAMS)
discussion
group
A Joint Meeting of GGPF (Golden Gate Polymer
Forum) and the Bay Area Mass Spectrometry (BAMS) discussion group
Tuesday November 13 , 2007
6:00 pm to 9:30 pm Michael's Restaurant at Shoreline Park
Mountain View, CA
Dinner reservations required by
noon on Friday November 9, 2007
Register early! As this is a joint meeting, seats are
limited!
"Structural Characterization of PEGylated Protein Therapeutics
using Gas-Phase Ion/Molecule Chemistry and Ion Mobility Tandem Mass
Spectrometry"
Paul Schnier
Department of Molecular Structure
Amgen, Thousand Oaks, CA
Abstract
Conjugation of polyethylene glycol (PEG) to a protein
therapeutic can safely improve pharmacokinetic properties, thus PEG and
its derivatives have found wide application in the biopharmaceutical
industry. PEGs used in these applications are typically polydisperse
and generally of high molecular weight, obfuscating direct analytical
characterization. Here, we demonstrate the use of ion mobility in the
separation of PEGs of different chain length, facilitating direct
measurement of MW and polydispersity of pegylated peptides/proteins.
Ion-molecule proton-transfer chemistry, in either the ion mobility cell
or source, can be used to enable charge stripping of the PEG ions in
order to further simplify the spectrum. Tandem mass spectrometry of the
mobility separated ions allows the site of PEGylation to be
unambiguously determined. Additionally, a novel functional analysis
algorithm to quantitatively compare the 3D topology of ion mobility MS
spectra has been developed. This approach allows for the direct
quantitation of low level impurities and degradation products present
in these samples; this methodology can be used in a high throughput
fashion to fingerprint heterogeneous PEGylated therapeutics. For these
applications, ion mobility coupled with mass spectrometry offers
several analytical advantages over mass spectrometry alone, including
enhanced dynamic range, increased sensitivity and specificity.
Speaker Background
Paul Schnier received his Ph.D. in Physical Chemistry from the
University of California, Berkeley, in 1998, followed a by
post-doctoral fellowship at the Oak Ridge National Laboratory. He has
worked in the biopharmaceutical industry for the last eight years, and
is currently Principal Scientist in the Department of Molecular
Structure at Amgen. His current research interests are focused on the
application of novel mass spectrometric, NMR, and biophysical
methodologies to drug discovery.
Meeting details
| Date: |
Tuesday
|
November 13,
2007
|
| Time: |
6:00 pm |
Social hour, registration (no-host cocktails) |
|
7:00 pm |
Dinner |
|
8:00 pm |
Presentation
|
| Dinner: |
featuring: |
Salmon |
|
|
Chicken Florentine
|
|
|
Grilled
Vegetable Brochette
|
| Cost: |
$30
|
BAMS members* |
|
$40
|
Non-members |
|
$60
|
BAMS membership plus dinner cost |
|
$20
|
Students only |
|
$300
|
2007 Corporate Sponsors |
|
|
Dinner reservations
required
by noon on Friday November 9, 2007
|
*Note: BAMS memberships are for the calendar year and should
be renewed with the first event you attend in 2007. Dues ($30.00)
may be paid while registering for dinner. If you
are
unsure of your Membership status, please email Tim Hawkins.
Maps &
directions
Michael's Restaurant at Shoreline Park
2960 N.Shoreline Blvd
Mountain View, CA
Google
Map of Michael's
Restaurant Phone: 650-962-1014
If you would like to
subscribe/unsubscribe or make changes to your email address in our
distribution
list, please email Yingying Huang.
Did you see or hear about the BAMS talk by Professor Murayama
entitled
"What is Mass?" What is it we are measuring after
all?
Check it out! Go to ctp.berkeley.edu
and click on "Popular Talks & Articles" for a QuickTime file.
As a service to our members, we post Employment
postings.
The San Francisco Bay Area Mass Spectrometry discussion group was
formed
in 1980 to provide a regular gathering for people interested in mass
spectrometry
and allied topics. BAMS currently has a membership of about 280
individual
and 20 corporate members, and meets 8-10 times per year for a midweek
dinner
and lecture. Meetings attract between 30 and 90 people, and are
held
at a restaurant or hotel in the bay area convenient for our
speaker.
We usually convene at 6:00 pm for cocktails, dinner at 7:00 pm, and
lecture
at 8:15 pm.
If you have suggestions for a meeting topic, or know of a prominent
mass spectrometrist who will be visiting the bay area, please contact
our
program
chair.
To become a member, please fill out and mail in a membership
form.
BAMS has been fortunate to have had many excellent
speakers in our history.
If you have suggestions for a meeting topic please contact our program
chair.
If you have questions or comments about BAMS, please contact one of
the BAMS officers.
Please thank our corporate sponsors who
help keep BAMS membership and dinner costs low.
Here are some internet links that may be of
interest to BAMS members.
If you have comments or suggestions for this web site, please email
me at hugh-gregg@llnl.gov
Last update: 9/12/2007