Abstract
Gaseous bio-ions have exhibited rich chemistry including reaction phenomenologies
such as proton transfer, hydrogen/deuterium exchange, nucleophilic substitution,
electron transfer, clustering, and, of course, unimolecular decomposition.
The latter reaction class is used routinely in bioanalytical applications
of mass spectrometry, including applications of ion trap mass spectrometry.
This presentation focuses on bi-molecular reactions involving ions derived
from the analyte molecule of interest and an oppositely charged reagent
species.
Particular emphasis in this talk is placed on the reactions of multiply-charged
bio- ions with singly-charged ions of opposite polarity. The capability
for such ion/ion reactions to manipulate bio-ion charge states can play
an important role in several bioanalytical applications of interest.
The focus of this presentation is on the use of charge state manipulation
via ion/ion chemistry for the analysis of polymer mixtures, primarily polypeptide
mixtures. The desired characteristics of the singly-charged reagent
will be discussed along with implications of the kinetics of ion/ion reactions
for bioanalysis with data presented to illustrate each point. Several
polypeptide mixtures of known composition have been studied along with
the electrospray behavior of each individual component observed in the
absence of the other components. These studies provide useful insights
into the extent to which the direct mixture analysis capabilities of electrospray
can be expanded by the use of gaseous ion/ion chemistry.
Background
Scott McLuckey received a bachelor's degree in chemistry from Westminster
College (New Wilmington, PA) in 1978 and a doctorate in chemistry in 1982
from Purdue University, working under the direction of Professor R.G. Cooks.
In 1983 he served as a post-doc at the FOM Institute for Atomic and Molecular
Physics, Biomolecular Physics Group, in Amsterdam. In late 1983,
he was awarded a Wigner Fellowship by Oak Ridge National Laboratory in
Oak Ridge, TN where he joined the Analytical Chemistry Division as a research
staff member. In January of 1992, he assumed the role of Head of
the Analytical Spectroscopy Section of the Chemical and Analytical Sciences
Division of Oak Ridge National Laboratory.
Scott's research pursuits have involved physical organic chemistry and
instrument development as they pertain to analytical mass spectrometry.
He and his colleagues, for example, have focused on instrumentation for
and reactions in mass spectrometry/mass spectrometry and ionization by
atmospheric sampling glow discharge, positron annihilation, and electrospray.
In recent years, much of this work has involved the quadrupole ion trap.
Heavy emphasis, in particular, has been placed on ion trap mass spectrometry
for the study of the chemistry of multiply-charged biopolymers.
Meeting details
| Date: | Monday | January 19, 1998 |
| Time: | 6:00 pm | Social hour, registration (no-host cocktails) |
| 7:00 pm | Dinner ($25 includes wine) | |
| 8:15 pm | Lecture (free, no reservations required) | |
| Dinner: | Choice of: | Pork Loin with Rosemary Sauce |
| Chicken Rosemary | ||
| Vegetarian Lasagna | ||
| includes | salad, strawberry short cake, wine, coffee | |
| Cost: | $25.00 | Reservations required |
| $10.00 | Students only |