Abstract
The attachment of a carbohydrate to a protein (glycosylation) is one
of the most common post-translational modifications encountered in eukaryotic
systems. It has been estimated that 60-90% of all mammalian proteins are
glycosylated at some point during their existence. These carbohydrate chains
play critical roles in numerous biological systems. Often these carbohydrate
side chains play a direct physiological role in a glycoprotein's biological
activity, while in other cases they maintain physical properties. Carbohydrate
side chains may also play a role in the immune response to glycoproteins,
thereby increasing the difficulty of obtaining glycoprotein therapeutics
that do not elicit an adverse immunological response. Consequently, structural
information concerning the carbohydrate chains attached to a glycoprotein
is of immense value, not to mention that it is often required for regulatory
review of new therapeutic agents.
This presentation will discuss our general approach for analyzing glycoproteins,
the development of new/improved procedures to aid in their characterization,
and some of the typical problems encountered, but rarely published, in
the mass spectrometric analysis of these samples.
Background
Ron Orlando received his BS from St. Mary's College of Maryland and
his Ph.D. in Analytical Chemistry in 1988 from the Department of Chemistry
and Biochemistry at the University of Delaware under the direction of Burnaby
Munson. After three years at the University of Maryland (half as
a postdoctoral research fellow for Catherine Fenselau and half as a research
associate), Ron became the Director of Mass Spectrometric Research and
Senior Scientist at Suntory Institute for Bioorganic Research in Osaka,
Japan. In 1993, he joined the faculty at the University of Georgia
and is currently an Associate Professor in both the Department of Biochemistry
& Molecular Biology (Complex Carbohydrate Research Center) and the
Department of Chemistry.
Meeting details
| Date: | Wednesday | August 25, 1999 |
| Time: | 6:00 pm | Social hour, registration (no-host cocktails) |
| 7:00 pm | Dinner ($25/members; $30/non-member includes wine) | |
| 8:00 pm | Presentation (free, no reservations required) | |
| Dinner: | Choice of: | Atlantic King Salmon and Rice Pilaf |
| Prime Rib of Beef and Rice Pilaf | ||
| Portobello Mushrooms and potatocakes | ||
| includes | California Mixed Green Salad, Rolls, and Kahlua Cheesecake, wine, coffee | |
| Cost: | $25.00 | BAMS members. Reservations required by noon Monday 8/23/99 |
| $30.00 | Non-members. Reservations required by noon Monday 8/23/99 | |
| $15.00 | Students only. Reservations required by noon Monday 8/23/99 |
The San Francisco Bay Area Mass Spectrometry discussion group was formed
in 1980 to provide a regular gathering for people interested in mass spectrometry
and allied topics. BAMS currently has a membership of about 280 individual
and 20 corporate members, and meets 8-10 times per year for a midweek dinner
and lecture. Meetings attract between 30 and 90 people, and are held
at a restaurant or hotel in the bay area convenient for our speaker.
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at 8:15pm.
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Last update: 8/16/99.