Abstract
The history of HPLC has seen a progression toward the use of columns packed with particles of decreasing size. Decreasing particle size has led to smaller values of the plate height and faster optimum velocities.  Due to pressure limitations of existing HPLC equipment this trend has translated, not into columns of increasing separation efficiency, but instead, into shorter columns offering much faster analysis times.  Using electric fields and electroosmotic flow to drive mobile phase through a packed capillary column (Capillary Electro-Chromatography, or CEC) is one way to use micron-sized particles in longer capillary columns. The use of much higher pressures (Ultra High Pressure Liquid Chromatography, or UHPLC) is another means to produce flow in such columns. Significant amounts of heat can be generated in pumping solvents at optimum velocities through such a highly restrictive bed of particles. In a column of conventional diameter (4.6 mm), this heat will result in both axial and radial temperature gradients, which lead to excessive band spreading. Packed capillary columns can be used to reduce this difficulty. The design and performance of a system for doing liquid chromatography in packed capillary columns at pressures in excess of 5,000 bar (75,000 PSI) will be described.
 
 

Abstract
Although the world has seen enormous development in analytical instrumentation over the past decades, the continuously increased interest for molecular knowledge in complex environments and dynamic processes gives us analytical challenges that are greater than ever before. Our contribution to this research area is to develop miniaturized liquid based analytical tools and chemometric algorithms in combination with mass spectrometric detection. Favorable combinations of pretreatment, separation and detection should always be considered, but this is especially valid when providing optimal ionization and desolvation conditions for liquid based separations coupled to mass spectrometry. Ultimate speed is achieved when analytes are separated by capillary electrophoresis or electrochromatography and mass analyzed using an orthogonal time-of-flight mass spectrometer with high-speed electronics. The enhanced selectivity attainable by liquid chromatography or supercritical fluid chromatography also makes these separation techniques advantageous tools as miniaturized high performance techniques.
 

Note:  This is a joint meeting with the California Separations Science Society, and all arrangements for the dinner and talks are being handled by CaSSS.  The social hour starts at 5:00pm (instead of the "normal" BAMS time of 6:00pm).  CaSSS has a registration policy of offering members reduced registration fees if a check (or credit card number) is received before 11/30/99 (walk-in, non-members cost is $40).  Receipts will be issued at the meeting.  See their web site at www.casss.org for more information.

Meeting details

Date:	Thursday	December 9, 1999
Time:	5:00 pm	Social hour, registration
	6:00 pm	Dinner (see the CaSSS web site for choices)
	7:00 pm	Presentations
Cost:	$30.00	BAMS members (reservations before 11/30/99)
	$15.00	Students only.

 

The San Francisco Bay Area Mass Spectrometry discussion group was formed in 1980 to provide a regular gathering for people interested in mass spectrometry and allied topics. BAMS currently has a membership of about 280 individual and 20 corporate members, and meets 8-10 times per year for a midweek dinner and lecture.  Meetings attract between 30 and 90 people, and are held at a restaurant or hotel in the bay area convenient for our speaker.  We usually convene at 6:00pm for cocktails, dinner at 7:00pm, and lecture at 8:15pm.
 

If you have suggestions for a meeting topic, or know of a prominent mass spectroscopist who will be visiting the bay area, please contact our program chair.
 

As a service to our members, we post Employment postings.

To become a member, please fill out and mail in a membership form.
BAMS has been fortunate to have had many excellent speakers in our short history.
If you have suggestions for a meeting topic please contact our program chair.
If you have questions or comments about BAMS, please contact one of the BAMS officers.
Please thank our corporate sponsors who help keep BAMS membership and dinner costs low.
Here are some internet links that may be of interest to BAMS members.
 

If you have comments or suggestions for this web site, please email me at hugh-gregg@llnl.gov

Last update: 11/19/99.